Morphometric determination of healthy young female's auricles / 中华医学美学美容杂志

Objective To investigate auricle index difference in Henan residents,and to provide an additional reference for otoplasty.Methods Subjects were made up 266 healthy young of Henan residents.Their height,weight,total head height,maximum head length,maximum head breadth,vertex to tragion height,physiognomic ear breadth,morphological ear breadth,physiognomic ear length and morphological ear length were measured and calculated.All the data were analyzed by SPSS 15.0 software.Results We obtained the means,standard deviation of each items,and data were treated statistically.Height (162.9±4.1) cm,weight (55.3±6.5) kg,total head height (18.6±1.0) cm,maximum head length (17.0±0.7) cm,maximum head breadth (14.1±0.8) cm,vertex to tragion height (11.9±0.7) cm,physiognomic ear length (6.2±0.4) cm,morphological ear breadth (4.9±0.6) cm,physiognomic ear breadth (3.3±0.3) cm and morphological ear length (2.9±0.3) cm were determined.The differences among the indices mentioned above were of statistical significance (P＜0.05);the indices of female subjects of different height were significantly different (P＜0.05);the indices of female subjects of different weight were also statistically different (P＜0.05).The auricle index subjects of different height and weight were of no stistical difference.(P＞0.05).Conclusions Subjects of different height and weight have different total head height;hence,the indices are related to body height and weight.Subjects of different height have different vertex to tragion height,length-height index of the head and breadth-height index of the head.This study has certain reference values on analyzing the characteristics of the human and auricle cosmetology of henan young females.

Determination and analysis of facial-cervical region, body height and weight index of college undergraduates / 中华医学美学美容杂志

Objective To investigate facial-cervical index difference in undergraduates of different height or weight in Han nationality to provide an additional reference for plastic surgery of facialcervical region.Methods Subjects consisted of 200 college undergraduates of Han nationality,aged 20 to 24 years,with average 22.9 ± 1.7,of whom 100 were male and 100 were female.Their height,weight,bizygomatic breadth,facial height,cervical height,neck circumference,neck breadth,shoulder width,cervical-mandibular angle,thorax-cervical angle,shoulder-cervical angle,pulvinar-cervical angle and back-cervical angle were measured and calculated.Results The differences among the indices mentioned above were of statistical significance (P＜0.05);the indices of female subjects of different height were significantly different (P＜0.05);the differences in indices of male subjects of different weight were of statistical significance (P＜0.05);the indices of female subjects of different weight were also statistically different (P＜0.05).The facial-cervical indices in both male and female subjects of different height or weight were of no stistical difference (P＞0.05).Conclusions Male subjects of different height or weight have different cervical height,cervical-mandibular angle,neck height and facial height indices are different;hence,the indices are related to body height;female subjects of different body height have different neck height.Subjects of different weight have different neck circumference,neck breadth,should breadth,shoulder-cervical angle and pulvinar-cervical angle.

Construction and identification of an RNA interference lentivirus vector targeting the Ras homology C gene of melanoma cells / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Melanoma has the highest mortality among all superficial malignant tumors. The poor prognosis is due to its high metastasis rate and the lack of therapeutic targets. As a molecular switch that controls tumor metastasis, Ras homology C (RhoC) has been correlated with tumor progression, especially tumor invasion and metastasis. However, little research has been done about the effects of RNA interference (RNAi) targeting RhoC on the invasion and metastasis of melanoma. In this study, we constructed an RNAi lentivirus vector targeting the RhoC gene of melanoma cells and identified its silencing effects on the RhoC gene.</p><p><b>METHODS</b>Based on the RhoC gene encoding information, three pGPU6/GFP/Neo-short hairpin (shRNA) plasmids were constructed. After detecting their silencing effects on the RhoC gene of A375 cells, the most effective pGPU6/GFP/Neo-shRNA plasmid was packed with lentivirus to construct the recombinant pLenti6.3-EGFP-453 targeting RhoC. The lentivirus vector was used to infect A375 cells, and then the expression of RhoC mRNA and protein were determined with real-time PCR and Western blotting.</p><p><b>RESULTS</b>The plasmids pGPU6/GFP/Neo-shRNA 336, pGPU6/GFP/Neo-shRNA 453, and pGPU6/GFP/Neo-shRNA 680 were constructed. After they were transfected into A375 cells, the expressions of RhoC mRNA and protein were 1.47 ± 0.26, 1.13 ± 0.16, 1.39 ± 0.11 and 70.98 ± 9.21, 50.67 ± 6.06, 65.77 ± 4.06, respectively. pGPU6/GFP/Neo-shRNA 453 was the most effective sequence, and was used to successfully construct the pLenti6.3-EGFP-453 lentiviral vector targeting RhoC. pLenti6.3-EGFP-453 was used to infect A375 cells. The expression of RhoC mRNA and protein were 1.05 ± 0.05 and 62.04 ± 15.86 in the lentivirus group, 4.21 ± 0.24 and 220.86 ± 24.07 in the negative lentivirus control group, and 4.63 ± 0.32 and 257.39 ± 12.30 in the normal control group respectively with the difference between the lentivirus group and the control groups being statistically significant (P < 0.05).</p><p><b>CONCLUSION</b>The successfully constructed pLenti6.3-EGFP-453 vector targeting the RhoC can effectively infect human melanoma A375 cells in vitro, and significantly inhibit the RhoC mRNA and protein expression.</p>

Measurement of hip and thigh profile in young females from the central plain area of China and its clinical significance / 中华医学美学美容杂志

Objective To investigate the relevant data and aesthetic standards of hip and thigh profile in young females from the central plain area of China, aiming to provide a credible reference for the clinical application. Methods Measurements were made in 551 young healthy females aged 16 to 21 years, including waist circumference, hip circumference, maximum thigh circumference, thigh length and the ratio between thigh length and height. Based on these data, the features of the hip and thigh profile were analyzed. Results Waist circumference, hip circumference, maximum thigh circumference, thigh length and the ratio between thigh length and height were ( 70. 74 ± 5.86 ), ( 87.16 ± 4. 19 ), ( 51.87 ± 3.27 ),(42.35 ±2.38) cm and (26. 1 ± 1.23) %, respectively. The hip and maximum thigh circumference showed significant difference between different height and weight groups. The thigh length and the ratio between thigh length and height were positively correlated with height. Conclusion The parameters of hip and thigh profile in young females from the central plain area are of significant values in providing quantitative references for the plastic surgery and evaluation of the operation,especially in liposuction.

Pathogenesis of keloids and its related factors of recurrence: a clinical analysis of 476 cases / 中华医学美学美容杂志

Objective To study what affect the incidence and recurrence of keloids. Methods A total of 476 cases were treated with surgery plus adjunctive radiation therapy, intraleisional injection of triamcinolone acetonaide acetate, or surgery plus postoperative topical medicine. The family history and other clinical data were analyzed, including patients' age, sex, the duration of keloids, initiating factors, location, size, treatment method, and recurrence of the keloids. Results Keloids occurred in both male and female with the rate of 1. 83 ∶ 1. 00, with a predominance in woman aged from 22 to 45 years old. Trauma and pierce were the most significant initiating factors. The recurrence was affected by patients family history, treatment methods and the location of keloids, and less affected by keloids size, patients' age and sex. Conclusions Both surgery following adjunctive radiation therapy and intraleisional Kenalog injection are useful methods, which are prior to surgery plus postoperative topical medicine. The keloids incidence has a preponderance in some patients with family history. Therefore,genetic research is important to identify the pathogenetic factors in the keloids.

Anthropometic measurements and analysis of abdominal profile in young health females / 中华医学美学美容杂志

Objective To study the aesthetic standard and parameters of abdominal profile in young females, in order to provide anatomic data for abdomino plasty and umbilicus reconstruction or transposition. Methods Measurements were conducted in 281 young health females, including height, weight, abdominal length, abdominal width and umbilicus location. All data were analyzed. Results The abdominal length, abdominal width, length from appendix ensiformis to omphalion, length from appendix ensiformis to middle point between appendix ensiformis and symphysion were (37. 93 ± 2. 15), (36. 98 ± 2. 71), (21. 10±1.80), and (18. 96±1. 08) cm, respectively. Abdominal length was positively correlated with height. Abdominal width, and the ratio between abdominal length and width were positively correlated with weight. The location of umbilicus was lower 2. 1 cm than middle point between appendix ensiformis and symphysion. Conclusion The abdominal profile is significant for clinical reference during abdomino plasty and umbilicus reconstruction or transposition.

Measurement and related factor analysis of leg length in young unmarried females / 中华医学美学美容杂志

Objective To investigate the aesthetic standard of leg length in young unmarried females.and to provide an additional reference for leg-plasty.Methods According to the data of the 531 young healthy women aged 17 to 20 years,the mean,standard of height,weight,hip circumference,lowaer limb length,leg length,leg girth,lower leg length-girth index,and leg girth index were calculated.Correlations between these parameters were analyzed by SPSS 15.0 software.Results Heightwas(163.22±4.03)cm,weight was(53.82±6.12)kg,hip circumference was (91.51±4.56)cm,whole leg was(75.95±2.97)cm,leg was(37.97±1.83)cm,length of leg maximal girth to tibial tuberosity(14.13±0.74)cm,length of leg medium girth to tibial tuberosity (18.99±0.92)cm,leg maximal girth(34.91±1.82)cm,leg minimal girth(20.30±1.06)cm,leg length to height ratio was 23.26±0.90,lower leg to whole leg ratio 50.01±1.95,leg maximal girth to hip circumference was 38.18±1.66,lower leg length-girth index was 92.12±5.86,leg girth index was 58.21±2.84.and the distance from leg maximal girth point to tibial tuberosity to leg length ratio was 37.26±1.78.Distance from leg maximal girth point to tibial tuberosity to leg length ratio and leg girth index had significant correlation with height,and hip circumference,but had no significant correlation with weight.Lower leg length-girth index had significant correlation with height,weight,and hip circumference.Conclusion These data have some reference value on analyzing the characteristics of human female leg cosmetology.

An immunohistochemical study on relationship between the changes of actin in cardiac and skeletal muscle cells and postmortem interval in rats / 中国法医学杂志

Objective To study the relationship between the changes of actin in cardiac muscle and skeletal myogen cells and postmortem interval in rats.Methods The changes of actin in cardiac muscle and skeletal myogen cells at different postmortem intervals were observed by immunohistochemical S-P method using anti-actin monoclonal antibody (HHF35)and analyzed by IBSA image analysis system.Results Within 54h postmortem,cardiac and skeletal muscles revealed depletion of HHF35 staining.The extent of HHF35-staining depletion increased with extension of postmortem interval.Statistical difference was significant in the data obtained by IBSA image analysis system (F cardiac muscle =588.27,F skeletal muscle =351.25,P